Downstream Processing of Natural Products: A Practical Handbook by Michael Verrall

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Downstream Processing of Natural Products: A Practical Handbook

by Michael Verrall

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Natural Products, broadly defined as high value chemical entities derived from plants or microbial sources, have been known and exploited for many years. In recent years, as the need for higher potency and predictability of such products has increased, more sophisticated concentration and isolation procedures have been developed. With the passage of time, such procedures have been rationalized in terms of scientific principles but, in general theory has followed behind practice, leading at any given time to an absence from the literature of methods which are truly state of the art. Downstream Processing of Natural Products, A Practical Handbook is a highly practical manual which addresses this issue and guides researchers and industrial workers through the many potential pitfalls of natural product isolation. The contributors to this volume, all of whom have wide practical experience in this field, present state of the art techniques and observations. The three main stages of natural product purification are covered, namely product release, capture, purification, both proteins and secondary metabolites are covered. There is special mention of the requirements of the regulatory authorities with respect to good manufacturing practice and practical guidance is given on scale up procedures and process scale instrumentation. It will provide essential practical guidance to all those involved in natural product isolation. This includes academic and industrial researchers, postgraduate students and technicians working in the biotechnology field. Broth Conditioning and Clarification. Cell Disruption. A Practical Approach, Solvent Extraction of Fermentation Broth, Chemically Assisted Solvent Extraction, Process Integration in Biotechnology, Displacement Chromatography, Affinity Chromatography, High-Performance Liquid Chromatography, Lyophilization, Instrumentation and Process Control, Scale-Up Considerations, GMP and Quality Control.ISBN-9788126538881

Pages : 372

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